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Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography

✍ Scribed by A.R. Buckpitt; D.E. Rollins; S.D. Nelson; R.B. Franklin; J.R. Mitchell

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 604 KB
Volume: 83
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90522-x

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✦ Synopsis

A quantitative method for the estimation of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen obtained from microsomal incubations has been developed using high-pressure liquid chromatography (HPLC). As little as 5 ng of the thiol conjugates of [ring-Tlacetaminophen can be measured with good precision from microsomal incubations with minimal sample preparation. High-pressure liquid chromatography offers distinct advantages over the standard separation techniques of thin-layer, paper, or gel filtration chromatography for the separation and quantitation of the polar thiol metabolites of acetaminophen.

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