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Analysis of Glutathione, Glutathione Disulfide, Cysteine, Homocysteine, and Other Biological Thiols by High-Performance Liquid Chromatography Following Derivatization by N-(1-Pyrenyl)maleimide

✍ Scribed by R.A. Winters; J. Zukowski; N. Ercal; R.H. Matthews; D.R. Spitz


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
658 KB
Volume
227
Category
Article
ISSN
0003-2697

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A method is described for the detection of glutathione (GSH) and glutathione disulfide (GSSG) based on a HPLC postcolumn reaction with ortho-phthalaldehyde (OPT) at pH 12 followed by fluorescence detection. Although similar methods have been reported, the high pH of the postcolumn reaction adds cons