Quantitative determination of phospholipids in amniotic fluid by high-performance liquid chromatography
β Scribed by T. Heinze; G. Kynast; J. W. Dudenhausen; C. Schmitz; E. Saling
- Book ID
- 112732231
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 519 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0009-5893
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π SIMILAR VOLUMES
## Abstract A method for the HPLC separation of phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylcholine (PC), and sphingomyelin (SPH) was achieved using five inβseries columns packed with LiChrosorb, Partisil, and ΞΌβPorasil adsorbents, a solvent mixture of chloroform/methanol/ammo
Quantitation of individual phospholipids sepamted by HPLC from tissue extracts by calorimetric analysis of phosphate was investigated. Elution of inorganic phosphate and breakthrough of lecithin were determined using radioisotopes. A substance which interfered with sample phosphate determinations wa