Quantitative determination of hemoglobin and cytochemical staining for peroxidase using 3,3′,5,5′-tetramethylbenzidine dihydrochloride, a safe substitute for benzidine

✍ Scribed by H.H. Liem; F. Cardenas; M. Tavassoli; M.B. Poh-Fitzpatrick; U. Muller-Eberhard

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 874 KB
• Volume: 98
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90157-x

✦ Synopsis

The quantitative determination of hemoglobin employing 3,3',5.5'-tetramethylbenzidine dihydrochloride (TMB-dl is described. This agent can also be used for staining myeloperoxidase-containing granules in granulocytes. TMB-d is at least as sensitive a reagent as benzidine but. unlike benzidine and diaminobenzidine. it is not a carcinogen.