Quantitative competitive (QC) PCR for quantification of porcine DNA
✍ Scribed by Christian Wolf; Jürg Lüthy
- Book ID
- 117496146
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 449 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0309-1740
No coin nor oath required. For personal study only.
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We frequently use competitive PCR in the plateau phase in quantifying DNA species with a small number of cells. However, the basic issues of this method are poorly understood. Here, first we analyze this method theoretically under a generalized condition that competitor and target DNA products accum
The enzymological principles of the polymerase chain reaction (PCR) and of the quantitative competitive PCR (QC-PCR) are developed, proposing a theoretical framework that will facilitate quantification in experimental methodologies. It is demonstrated that the specificity of the QC-PCR, i.e. the rat