✦ LIBER ✦

Quantitative Analysis of Residual Detergent in Proteoliposomes by Fourier Transform Infrared Spectroscopy

✍ Scribed by A.M.A. Pistorius; F.M.A.H.S. Stekhoven; P.H.M. Boveegeurts; W.J. Degrip

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 356 KB
Volume: 221
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1994.1376

No coin nor oath required. For personal study only.

✦ Synopsis

An infrared spectroscopic method has been developed to quantify the residual amount of detergent present in membranes, e.g., after reconstitution of a membrane protein in phospholipids. A detergent-specific band is selected in the ir spectrum and the intensity is ratioed against a phospholipid-specific band (around 1235 cm-1, vas (P = O)). This ratio shows a linear relation with the molar ratio of detergent of phospholipid, down to as low as 1 to 10. The new method is illustrated in two case studies, viz., tris(hydroxyethyl)ammonium cholate in Na+/K(+)-ATPase proteoliposomes and n-dodecyl-beta-D-maltoside in rhodopsin proteoliposomes.

📜 SIMILAR VOLUMES

Quantitative Analysis of Norditerpenoid

Quantitative Analysis of Norditerpenoid Alkaloids in Larkspur (Delphinium spp.) by Fourier Transform Infrared Spectroscopy

✍ Dale R. Gardner; Gary D. Manners; Michael H. Ralphs; James A. Pfister 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 167 KB 👁 2 views

A Fourier transform infrared (FT-IR) spectroscopic method for measuring norditerpenoid alkaloids in larkspurs was developed. Alkaloids were isolated from dry plant material using a simplified extraction procedure and quantitated using FT-IR. Calibration of the method was made from selected larkspur

Analysis of human tear fluid by Fourier

Analysis of human tear fluid by Fourier transform infrared spectroscopy

✍ Yasushi Nagase; Satoshi Yoshida; Kenji Kamiyama 📂 Article 📅 2005 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 247 KB 👁 1 views

## Abstract The purpose of this research is to find some useful spectroscopic factors in human tear fluid contents to monitor diurnal changes of the physicochemical ocular conditions noninvasively. All tear fluid samples were collected with glass microcapillary tubes from both eyes of three donors

Thiocyanate Levels in Human Saliva: Quan

Thiocyanate Levels in Human Saliva: Quantitation by Fourier Transform Infrared Spectroscopy

✍ Christian P. Schultz; M.Khalique Ahmed; Colin Dawes; Henry H. Mantsch 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 127 KB

Quantitative determination of amorphous

Quantitative determination of amorphous cyclosporine in crystalline cyclosporine samples by Fourier transform infrared spectroscopy

✍ Vittorio Bertacche; Elena Pini; Riccardo Stradi; Fabio Stratta 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 129 KB

The purpose of this study is the development of a quantification method to detect the amount of amorphous cyclosporine using Fourier transform infrared (FTIR) spectroscopy. The mixing of different percentages of crystalline cyclosporine with amorphous cyclosporine was used to obtain a set of standar

In situ study of photopolymerization by

In situ study of photopolymerization by fourier transform infrared spectroscopy

✍ Atsushi Udagawa; Fumio Sakurai; Tatsuo Takahashi 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 563 KB

Quantitative determination of peroxide v

Quantitative determination of peroxide value in thermally oxidized palm olein by Fourier transform infrared spectroscopy

✍ G. Setiowaty; Y. B. Che Man; S. Jinap; M. H. Moh 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 71 KB 👁 2 views

A simple and rapid quantitative method to determine peroxide value (PV) of palm olein has been developed using transmittance Fourier transform (FT) IR spectroscopy. Palm olein was oxidized and blended with unoxidized palm olein to generate samples with PVs in the range 3.52-9.86. These samples were