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Quantitative Analysis of Norditerpenoid Alkaloids in Larkspur (Delphinium spp.) by Fourier Transform Infrared Spectroscopy

✍ Scribed by Dale R. Gardner; Gary D. Manners; Michael H. Ralphs; James A. Pfister

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 167 KB
Volume: 8
Category: Article
ISSN: 0958-0344
DOI: 10.1002/(sici)1099-1565(199703)8:2<55::aid-pca338>3.0.co;2-q

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✦ Synopsis

A Fourier transform infrared (FT-IR) spectroscopic method for measuring norditerpenoid alkaloids in larkspurs was developed. Alkaloids were isolated from dry plant material using a simplified extraction procedure and quantitated using FT-IR. Calibration of the method was made from selected larkspur samples with known alkaloid levels determined by high pressure liquid chromatography and gravimetric methods. Thirty-nine samples from three different larkspur species (Delphinium barbeyi, D. glaucescens, and D. occidentale) were analysed to demonstrate the ability of the FT-IR method to measure toxic and total alkaloid concentrations in these plants.

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