𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Quantitative Analysis of Pulmonary Surfactant Proteins B and C

✍ Scribed by Martin van Eijk; Catharina G.M.De Haas; Henk P. Haagsman

Book ID
102560062
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
118 KB
Volume
232
Category
Article
ISSN
0003-2697

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✦ Synopsis

A procedure for the quantification of surfactant protein B (SP-B) and surfactant protein C (SP-C) in surfactant preparations is described. After butanol extraction of surfactant, the resulting extract is separated by Sephadex LH-60 HPLC, using an elution fluid which consists of 30% dichloromethane, 65% methanol, and 5% 100 mM HCL. SP-B and SP-C are detected by absorbance at 228 nm and quantified using standard stock solutions. Run length is approximately 70 min. Detection limits are 1 microgram SP-B and 4 micrograms SP-C.

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A Quantitative Method for Detecting Surf
A Quantitative Method for Detecting Surfactant-Associated Protein C in Pulmonary Surfactant
✍ R. Qanbar; F. Possmayer πŸ“‚ Article πŸ“… 1994 πŸ› Elsevier Science 🌐 English βš– 704 KB

We describe a chemical method for the detection and quantification of surfactant protein C (SP-C), a palmitoylated \(3.5-\mathrm{kDa}\) protein, based on the release of freesulfhydryl groups upon depalmitoylation. SP-C was purified from calf lung surfactant extract by gel filtration on LH-20 and LH-