𝔖 Bobbio Scriptorium
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Phospholipid and protein analysis of pulmonary surfactant

✍ Scribed by R. C. Cottrell; I. A. Herød; D. Bayley; R. Purchase; K. Miller


Book ID
112484357
Publisher
Springer
Year
1983
Tongue
English
Weight
453 KB
Volume
3
Category
Article
ISSN
0144-8463

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A procedure for the quantification of surfactant protein B (SP-B) and surfactant protein C (SP-C) in surfactant preparations is described. After butanol extraction of surfactant, the resulting extract is separated by Sephadex LH-60 HPLC, using an elution fluid which consists of 30% dichloromethane,