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Quantitative analysis of iduronic acid and glucuronic acid in sulfated galactosaminoglycuronans by gas chromatography

✍ Scribed by Sadako Inoue; Mariko Miyawaki

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
681 KB
Volume
65
Category
Article
ISSN
0003-2697

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✦ Synopsis

A 'gas chromatographic method for the quantitative analysis of iduronic acid and glucuronic acid in sulfated glycosaminoglycans (acid mucopolysaccharides, AMPS) of the type of dermatan sulfate has been developed. Methanolysis and deamination-methanolysis were used for the depolymerization of AMPS. Both iduronic acid and glucuronic acid were stable under the methanolytic conditions. More than 90% of the uranic acids was recovered by the present technique from dermatan sulfates and chondroitin sulfate A. The method has been applied to the analysis of the relative proportions of iduronic acid and ghtcuronic acid in dermatan sulfate fractions from various mammalian tissues. 164

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