✍ Scribed by Anu Suomalainen; Ann-Christine Syvänen
No coin nor oath required. For personal study only.
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## Abstract Rapid diagnosis of human adenovirus (HAdV) infections was achieved by PCR in the recent years. However, conventional PCR has the risk of carry‐over contamination due to open handling with its products, and results are only qualitative. Therefore, a quantitative “real‐time” PCR with cons
The presence of allelic sequence variations in DNA fragments can be easily detected by measuring the extent of DNA strand exchange between test double-stranded PCR products (target) and labeled standard double-stranded PCR products (probe). Under selected hybridization conditions, sequences identica
## Abstract Human cytomegalovirus (HCMV) infections and anti‐HCMV treatment are usually monitored by measuring pp65 antigenemia. This method is time‐consuming, labour‐intensive and requires skilled operators. We have compared results obtained using real‐time Light Cycler™ quantitative PCR (QPCR) an