The analysis of bile acids by spectrophotometric methods is often associated with significant errors from contaminating compounds (1, 2). Furthermore, the response of dihydroxy-and trihydroxycholanic acids to various calorimetric and spectrophotometric methods is variable (3). Partition chromatograp
Quantitative analysis of bile acids in human gallstone and serum by gas-liquid chromatography
β Scribed by Mizuta, T. ;Nagamitsu, S.
- Publisher
- Springer-Verlag
- Year
- 1968
- Tongue
- English
- Weight
- 67 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0435-1339
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## Abstract The proposed combination of a rather simple procedure for sample preparation with capillary gasβliquid chromatography using a barium carbonate/polyethyleneglycol 20,000 column and a Grobβtype onβcolumn injector permits measurement of bile acids in serum with high separation efficiency,
Several investigators have reported that ascorbic acid can be analyzed by gas-liquid chromatography (GLC) following conversion of the parent compound to its trimethylsilyl ether (l-5). We have applied this procedure to the analysis of ascorbic acid in tissues and in this report describe the techniqu