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Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion

โœ Scribed by Pal, Sukdeb; Kim, Min Jung; Song, Joon Myong


Book ID
111692335
Publisher
Royal Society of Chemistry
Year
2008
Tongue
English
Weight
375 KB
Volume
8
Category
Article
ISSN
1473-0197

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Using a fluorescence-based method, we have determined the number of thiol-derivatized single-stranded oligonucleotides bound to gold nanoparticles and their extent of hybridization with complementary oligonucleotides in solution. Oligonucleotide surface coverages of hexanethiol 12-mer oligonucleotid