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Quantitation of multidrug resistant MDR1 transcript in acute myeloid leukaemia by non-isotopic quantitative cDNA-polymerase chain reaction

โœ Scribed by M. P. A. Lyttelton; S. Hart; K. Ganeshaguru; A. V. Hoffbrand; A. B. Mehta


Book ID
114713590
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
685 KB
Volume
86
Category
Article
ISSN
0007-1048

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Quantitative analysis of human multidrug
โœ Hiroyuki Kobayashi; Yuzuru Takemura; Hayato Miyachi; Masaki Kawabata; Shigehisa ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 124 KB ๐Ÿ‘ 2 views

We have established competitive reverse transcriptase polymerase chain reaction (RT-PCR) assay for the quantification of MDR1 mRNA encoding P-glycoprotein (P-gp) by analyzing leukemia sublines of MOLT-3 with various expression of MDR1. The expression was quantified by simultaneous RT-PCR of cellular