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Detection of the multidrug resistance gene (MDR-1) expression by non-radioactive quantitative polymerase chain reaction

✍ Scribed by Lion, T.; Henn, T.; Gaiger, A.; Karlic, H.; Gadner, H.


Book ID
122615256
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
182 KB
Volume
29
Category
Article
ISSN
0959-8049

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We have established competitive reverse transcriptase polymerase chain reaction (RT-PCR) assay for the quantification of MDR1 mRNA encoding P-glycoprotein (P-gp) by analyzing leukemia sublines of MOLT-3 with various expression of MDR1. The expression was quantified by simultaneous RT-PCR of cellular