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Quantitation of free and conjugated bile acids in human feces using a high-pressure liquid chromatography counterion method

✍ Scribed by L.R. Ferguson; G.W. Rewcastle; J.M. Lello; P.G. Alley; R.N. Seelye

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 620 KB
Volume: 143
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90671-7

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✦ Synopsis

A method has been developed for extraction and purification of the major bile acids in human feces, and for their quantative estimation using high-pressure liquid chromatography. Freeze-dried fecal material was extracted with alkaline ethanol and, after removal of neutral steroids, was subjected to thin-layer chromatography, followed by reversed-phase Crs silica cartridge (SepPak) purification. The mixture was further separated into free, and glyco and tauro conjugates by ion-exchange chromatography. Subsequent resolution of individual bile acids was accomplished by HPLC using a counterion pairing method. o 1984 Academic PWSS. hc.

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