A novel method for the separation and quantitation of benzene metabolites using high-pressure liquid chromatography

✍ Scribed by William F. Greenlee; John P. Chism; Douglas E. Rickert

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 312 KB
• Volume: 112
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90307-9

✦ Synopsis

A method for the separation of benzene metabolites using reverse-phase high-pressure liquid chromatography is described. The antoxidant, ascorbic acid is added to an aqueous mixture of 1,2&benzenetriol, hydroquinone, catechol, and phenol, to prevent autooxidation. The eluting solvents are equilibrated with nitrogen, degassed, and maintained under a nitrogen atmosphere during the analysis. A highly resolved and reproducible profile of the metabolites is achieved under these conditions. This method should prove useful in a number of pharmacokinetic studies where the biotransformation of the parent compound to autooxidizable species such as polyphenols and quinones precludes analysis under aerobic conditions.

1,2,4-Benzenetriol, hydroquinone, phenol (PHE), and ascorbic acid were purchased from Aldrich Chemical Company (Milwaukee, Wise.). Catechol (CA) was obtained from Sigma Chemical Company (St. Louis, MO.). Male Fischer 344 rats were purchased from Charles River Breeding Labs (Wilmington, Mass.).

An hplc and data analysis system was assembled using the following components (Waters Associates, Inc., Milford, Mass.