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Quantitation of fluorescence energy transfer between cell surface proteins via fluorescence donor photobleaching kinetics

โœ Scribed by Young, R.M.; Arnette, J.K.; Roess, D.A.; Barisas, B.G.


Book ID
119410268
Publisher
Biophysical Society
Year
1994
Tongue
English
Weight
742 KB
Volume
67
Category
Article
ISSN
0006-3495

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Background: Fluorescence resonance energy transfer (FRET) is a powerful technique for measuring molecular interactions at ร…ngstrom distances. We present a new method for FRET that utilizes the unique spectral properties of variants of the green fluorescent protein (GFP) for large-scale analysis by f