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Fluorescence resonance energy transfer-based technologies in the study of protein–protein interactions at the cell surface

✍ Scribed by Víctor Fernández-Dueñas; Javier Llorente; Jorge Gandía; Dasiel O. Borroto-Escuela; Luigi F. Agnati; Carla I. Tasca; Kjell Fuxe; Francisco Ciruela


Book ID
118507062
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
783 KB
Volume
57
Category
Article
ISSN
1046-2023

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Background: Fluorescence resonance energy transfer (FRET) is a powerful technique for measuring molecular interactions at Ångstrom distances. We present a new method for FRET that utilizes the unique spectral properties of variants of the green fluorescent protein (GFP) for large-scale analysis by f