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Quantitation of eukaryotic topoisomerase I reactivity with DNA. Preferential cleavage of supercoiled DNA

โœ Scribed by Mark T. Muller

Book ID
119119395
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
455 KB
Volume
824
Category
Article
ISSN
0167-4781

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Quantitation of supercoiled DNA cleavage
Quantitation of supercoiled DNA cleavage in nonradioactive DNA: Application to lonizing radiation and synthetic endonuclease cleavage
โœ Betsy M. Sutherland; Paula V. Bennett; Kim Conlon; Gary A. Epling; John C. Suthe ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 989 KB

Quantitation of the conversion of nonradioactive supercoiled DNA to its open circular or linear forms on ethidium-stained electrophoretic gels has been difficult because of differential binding of ethidium to supercoiled DNA vs other forms under different conditions and the nonlinear response of pho