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Quantitation of DNA and RNA in crude tissue extracts by flow injection analysis

✍ Scribed by Elaine M. Caldarone; Lawrence J. Buckley

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
499 KB
Volume
199
Category
Article
ISSN
0003-2697

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✦ Synopsis

An automated two-dye flow injection analysis system to quantitate DNA and RNA in crude extracts of tissues is described. The method uses the fluorochrome dyes ethidium bromide and Hoechst 33258. DNA concentration is determined directly from its fluorescence in Hoechst dye. RNA is estimated from fluorescence in ethidium bromide after subtraction of the fluorescence due to DNA. This method has several advantages: a simple extraction procedure, a low detection limit (0.01 micrograms DNA and 0.10 micrograms RNA), automation, and a high sample throughput.

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