## Abstract Human cytomegalovirus (HCMV) infections and anti‐HCMV treatment are usually monitored by measuring pp65 antigenemia. This method is time‐consuming, labour‐intensive and requires skilled operators. We have compared results obtained using real‐time Light Cycler™ quantitative PCR (QPCR) an
Quantitation of cytomegalovirus (hCMV) DNA and β-actin DNA by duplex real-time fluorescence PCR in solid organ (liver) transplant recipients
✍ Scribed by Joachim Hänfler; KarlAnton Kreuzer; Katja Laurisch; Nada Rayes; Peter Neuhaus; ChristianAndreas Schmidt; Helmut Oettle
- Publisher
- Springer-Verlag
- Year
- 2003
- Tongue
- English
- Weight
- 312 KB
- Volume
- 192
- Category
- Article
- ISSN
- 0300-8584
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## Abstract After bone marrow (BM) or solid‐organ (SO) transplantation viremic __Cytomegalovirus__ (CMV) infection is observed frequently. Quantitative assay of CMV in blood helps the management of this clinical condition. In the present report, 83 samples from 39 solid organ recipients, three CMV
The polymerase chain reaction (PCR) for cytomegalovirus (CMV) DNA quantitation provides sensitive and specific data for detecting CMV as well as monitoring the infection and determining the appropriate antiviral strategy. A recently introduced real-time PCR assay for CMV DNA quantitation was applied