Quantitation of Cyclic Nucleotides in Biological Samples by Negative Electrospray Tandem Mass Spectrometry Coupled to Ion Suppression Liquid Chromatography

✍ Scribed by Erwin Witters; Luc Roef; Russell P. Newton; Walter Van Dongen; Eddy L. Esmans; Henri A. Van Onckelen

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 640 KB
• Volume: 10
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19960131)10:2<225::aid-rcm469>3.0.co;2-j

✦ Synopsis

Measurement of cyclic nucleotides by electrospray ionization mass spectrometry constitutes an unequivocal

quantitative and qualitative technique superior to the established estimation methods of UV spectroscopy, radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA) and other mass spectrometric ionization techniques such as electron impact, thermospray and fast-atom bombardment. Optimal conditions for negative-ion electrospray tandem mass spectrometry showed a linear detection range using solutions with a concentration ranging from lo-' and SxlO-'M with an absolute detection limit of lOOfmol injected.

Measurement of diagnostic product ions derived from low-energy collision-activated dissociation of the [M -H]-

ions provided unambiguous identification. Quantitation of cyclic nucleotides present in biological samples in the femtomole region, needed further purification using on-line ion suppression high-performance liquid chromatography in order to avoid reduced sensitivity caused by matrix quenching.