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QUANTITATION OF [3H]THYMIDINE UPTAKE BY STIMULATED HUMAN LYMPHOCYTES

โœ Scribed by J. J. O'Leary; C. Mehta; D. J. Hall; A. Rosenberg

Book ID
114715356
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
590 KB
Volume
13
Category
Article
ISSN
0960-7722

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Mouse spleen lymphocyte (SL) cells show a three to four-fold increase in 3 H-thymidine incorporation when incubated in tumour cell media, or in media containing tumour cell cytosol. Agarose gel chromatography of both 3 H-thymidine-labelled tumour cell media and cytosol shows a sharp peak of DNA-asso