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Technical aspects of low 3H-thymidine incorporation by mitogen stimulated canine peripheral blood lymphocytes in vitro

✍ Scribed by F. Kristensen; C. Walker; B. Kristensen; M. Vandevelde; A.L. De Weck


Book ID
119098775
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
464 KB
Volume
3
Category
Article
ISSN
0165-2427

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Mouse spleen lymphocyte (SL) cells show a three to four-fold increase in 3 H-thymidine incorporation when incubated in tumour cell media, or in media containing tumour cell cytosol. Agarose gel chromatography of both 3 H-thymidine-labelled tumour cell media and cytosol shows a sharp peak of DNA-asso