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Quantifying Protein–Protein Interactions Within Noncovalent Complexes Using Electrospray Ionization Mass Spectrometry

✍ Scribed by Boeri Erba, Elisabetta; Barylyuk, Konstantin; Yang, Yang; Zenobi, Renato


Book ID
121507568
Publisher
American Chemical Society
Year
2011
Tongue
English
Weight
899 KB
Volume
83
Category
Article
ISSN
0003-2700

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A novel mass spectrometry-based methodology using electrospray ionization (ESI) is described for the detection of protein-protein [interferon (IFN)-γ dimer] and protein-ligand [ras-guanosine diphosphate (GDP)] noncovalent interactions. The method utilizes ESI from aqueous solution at appropriate pH.