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Quantification of green fluorescent protein in cellular supernatant by capillary electrophoresis with laser-induced fluorescence detection for measurement of cell death

โœ Scribed by Kristian E. Swearingen; Wendy P. Loomis; Benjamin Kehimkar; Brad T. Cookson; Norman J. Dovichi

Book ID
116904248
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
594 KB
Volume
81
Category
Article
ISSN
0039-9140

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Detection of Aequorea victoria Green Flu
Detection of Aequorea victoria Green Fluorescent Protein by Capillary Electrophoresis Laser Induced Fluorescence Detection
โœ Douglas B. Craig; Jerome C. Y. Wong; Norman J. Dovichi ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 45 KB ๐Ÿ‘ 2 views

Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laserinduced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 ฯซ 10 ฯช 12 M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.