Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laserinduced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 ฯซ 10 ฯช 12 M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.
โฆ LIBER โฆ
Detection of Green Fluorescent Protein in a Single Bacterium by Capillary Electrophoresis with Laser-Induced Fluorescence
โ Scribed by Turner, Emily H.; Lauterbach, Kevin; Pugsley, Haley R.; Palmer, Vanessa R.; Dovichi, Norman J.
- Book ID
- 126306805
- Publisher
- American Chemical Society
- Year
- 2007
- Tongue
- English
- Weight
- 81 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0003-2700
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## Determination of phycobiliproteins by capillary electrophoresis with laser-induced fluorescence detection Phycobiliproteins are derived from the photosynthetic apparatus of cyanobacteria and eukaryotic algae. They are composed of a protein backbone to which linear tetrapyrrole chromophores are