Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay

✍ Scribed by Makito Tanaka; Yoshiyuki Takahashi; YinYan Xu; Nao Yoshida; Ayami Yoshimi; Itzel Bustos Villalobos; Asahito Hama; Nobuhiro Nishio; Hirokazu Hidaka; Yue Wang; Hiroshi Yagasaki; Seiji Kojima

• Book ID: 104040592
• Publisher: Elsevier Science
• Year: 2008
• Tongue: English
• Weight: 516 KB
• Volume: 32
• Category: Article
• ISSN: 0145-2126
• DOI: 10.1016/j.leukres.2007.11.016

✦ Synopsis

Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a (3)H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.