Quantification of gliadin levels to the picogram level by flow cytometry
β Scribed by Rosanna Capparelli; Ilaria Ventimiglia; Luigi Longobardo; Domenico Iannelli
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 171 KB
- Volume
- 63A
- Category
- Article
- ISSN
- 0196-4763
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Prior to flow cytometric analysis, the cells were fixed in 70% ethanol and stained with a DNAspecific dye. After 1 h of ampicillin incubation, the light scattering and fluorescence intensities of the susceptible cells increased 4.3-fold and 5-fold, respectively, but remained about constant for the r
The use of flow cytometry (FCM) to quantitatively analyze intracellular compounds is studied. FCM is a very useful technique for individual cell studies in microbial systems, and gives access to information which cannot be obtained in any other way. Nevertheless, it provides data in arbitrary units,