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Quantification of D-Asp and D-Glu in rat brain and human cerebrospinal fluid by microchip electrophoresis

✍ Scribed by Yong Huang; Ming Shi; Shulin Zhao

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
408 KB
Volume
32
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A microchip electrophoresis (MCE) method with LIF detection was presented for quantification of D‐aspartic acid (D‐Asp) and D‐glutamate (D‐Glu) in biological samples. D‐Asp and D‐Glu were determined after precolumn derivatization with FITC. The chiral separation was performed on a glass/PDMS hybrid microfluidic chip using γ‐CD as chiral selector in the running buffer. High sensitive detection was obtained by the LIF detection. The LODs (S/N = 3) for D‐Asp and D‐Glu were 6.0×10^–8^ and 4.0×10^–8^ M, respectively. Using this method, the levels of D‐Asp and D‐Glu in rat brain and human cerebrospinal fluid (CSF) were determined.

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