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Quantification of CBFβ/MYH11 fusion transcript by Real Time RT-PCR in patients with INV(16) acute myeloid leukemia

✍ Scribed by Marcucci, G; Caligiuri, M A; Döhner, H; Archer, K J; Schlenk, R F; Döhner, K; Maghraby, E A; Bloomfield, CD


Book ID
110054925
Publisher
Nature Publishing Group
Year
2001
Tongue
English
Weight
407 KB
Volume
15
Category
Article
ISSN
0887-6924

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Detection and quantification of CBFB/MYH
✍ Jürgen Krauter; Wolf Hoellge; Mike Peter Wattjes; Stefan Nagel; Olaf Heidenreich 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 156 KB

We used a newly established real-time RT-PCR assay for the quantification of the leukemia-specific CBFB/MYH11 transcripts in inv( 16)-positive acute myeloblastic leukemia. CBFB/MYH11 could be quantified over a five log range, with a detection limit of 10 molecules of a CBFB/MYH11 plasmid and a 1:10