Quantification of branched-chain α-keto acids as quinoxalinols: importance of excluding oxygen during derivatization

The α-keto acids of branched-chain amino

The α-keto acids of branched-chain amino acids: Simplified derivatization for physiological samples and complete separation as quinoxalinols by packed column gas chromatography

✍ Hans P. Schwarz; Irene E. Karl; Dennis M. Bier 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 585 KB

By use of a relatively new mixed stationary phase, complete separation of the branchedchain cy-keto acids as O-trimethylsilyl-quinoxalinol derivatives is achieved within 10 min by packed column gas chromatography. Precise quantification of less than 5 nmol of cr-keto acids in biological samples is p