The α-keto acids of branched-chain amino acids: Simplified derivatization for physiological samples and complete separation as quinoxalinols by packed column gas chromatography
✍ Scribed by Hans P. Schwarz; Irene E. Karl; Dennis M. Bier
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 585 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
By use of a relatively new mixed stationary phase, complete separation of the branchedchain cy-keto acids as O-trimethylsilyl-quinoxalinol derivatives is achieved within 10 min by packed column gas chromatography. Precise quantification of less than 5 nmol of cr-keto acids in biological samples is possible. In small aqueous samples the a-keto acids are directly derivatized without prior purification. Plasma need only be deproteinized by perchlorate and neutralized before derivatization. Average relative precision for determination of the three main branched-chain cy-keto acids is f 5.8%.