Quality control of radiolabelled biochemicals by field desorption mass spectrometry
β Scribed by H.-R. Schulten; W. D. Lehmann
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 473 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1076-5174
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This paper shows the first application of field desorption-tandem mass spectrometry (FD-MS/MS) in a hybrid sector, orthogonal acceleration time-of-flight mass spectrometer. This instrumentation has significant advantages in detection limits for MS/MS experiments over four sector mass spectrometers.
Leu-enkephalin is quantified down to 30 ng (52 pmol) by means of field desorption mass spectrometry. Extensive chemical derivatization (acetylation, permethylation) is avoided. Linearity is obtained from 30 to 900 ng.
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