𝔖 Bobbio Scriptorium
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Quality control by the ribosome following peptide bond formation

✍ Scribed by Zaher, Hani S.; Green, Rachel

Book ID
109897897
Publisher
Nature Publishing Group
Year
2008
Tongue
English
Weight
499 KB
Volume
457
Category
Article
ISSN
0028-0836

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Effect of spermine on peptide-bond forma
Effect of spermine on peptide-bond formation, catalyzed by ribosomal peptidyltransferase
✍ Dimitrios L. Kalpaxis; Denis Drainas πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 619 KB

The effect of spermine on the binding of AcPhe-tRNA to poly(U)-programmed ribosomes (step 1) and on the puromycin reaction (step 2) has been studied in a cell-free system, derived from E. coli. In the absence of ribosomal wash (FWR fraction) and at suboptimal concentration of Mg++ (6 mM), spermine s