A number of different procedures have been developed for use with matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) for the analysis of non-covalent protein-protein complexes. These include use of specific matrix and laser combinations, accumulation of "first shot" spectra, mo
Qualitative Determination of Specific Protein Glycation Products by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Peptide Mapping
β Scribed by Humeny, Andreas; Kislinger, Thomas; Becker, Cord-Michael; Pischetsrieder, Monika
- Book ID
- 126929352
- Publisher
- American Chemical Society
- Year
- 2002
- Tongue
- English
- Weight
- 101 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0021-8561
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Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has been employed for the evaluation of the glycation level of IgG from healthy subjects and also from well-and badly-controlled diabetic patients. The measurements have been performed on untreated plasma protein fractions. The da
While electrospray (ESI) mass spectrometry has already established its potential for the characterization of noncovalent protein complexes, matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) seemed not to be applicable hitherto because of limitations in matrix chemistry and sam