✦ LIBER ✦

Evaluation of IgG glycation levels by matrix-assisted laser desorption/ionization mass spectrometry

✍ Scribed by Annunziata Lapolla; Domenico Fedele; Rosaria Aronica; Massimo Garbeglio; Martina D'Alpaos; Roberta Seraglia; Pietro Traldi

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 157 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199708)11:12<1342::aid-rcm972>3.0.co;2-t

No coin nor oath required. For personal study only.

✦ Synopsis

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has been employed for the evaluation of the glycation level of IgG from healthy subjects and also from well-and badly-controlled diabetic patients. The measurements have been performed on untreated plasma protein fractions. The data obtained have shown that a clear mass increase, originating from non-enzymatic glycation processes, is observed in the case of diabetic patients: for well-controlled ones it is in the range 512-1565 Da, while it becomes 827-4270 Da for badly-controlled diabetic patients. This approach indicates that MALDI mass spectrometry is a highly specific tool that can be employed in the metabolic control of diabetic patients and in studies relating the IgG glycation level to possible immunological impairment.

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