Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular bea
β¦ LIBER β¦
Quadruplex real-time polymerase chain reaction assay for molecular diagnosis of Y-chromosomal microdeletions
β Scribed by Qiwei Guo; Fenghua Lan; Liangpu Xu; Yu Jiang; Li Xiao; Hailong Huang; Yulin Zhou
- Book ID
- 116480292
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 281 KB
- Volume
- 97
- Category
- Article
- ISSN
- 1556-5653
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## Abstract To improve timely anteβmortem human rabies diagnosis, methods to detect viral RNA by TaqManβbased quantitative reverse transcriptase polymerase chain reactions (qRTβPCRs) have been developed. Three sets of two primers and one internal dualβlabeled probe for each primer set that target d