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Pyruvate carboxylase as a model for oligosubstituted enzyme-ligand conjugates in homogeneous enzyme immunoassays

✍ Scribed by Daunert, Sylvia.; Payne, Bryan R.; Bachas, Leonidas G.


Book ID
126436951
Publisher
American Chemical Society
Year
1989
Tongue
English
Weight
665 KB
Volume
61
Category
Article
ISSN
0003-2700

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A homogeneous enzyme immunoassay for lipoic acid was developed by using an enzyme-ligand conjugate containing only one ligand per enzyme subunit. Theoretical studies have shown that the traditional use of multisubstituted enzyme-ligand conjugates has limited the detection limits and sensitivity obta