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A homogeneous enzyme immunoassay with avidin-ligand conjugate as the enzyme-modulator

✍ Scribed by Cathy Bacquet; D.Y. Twumasi

Book ID
107712015
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
324 KB
Volume
136
Category
Article
ISSN
0003-2697

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Homogeneous enzyme immunoassay for lipoic acid based on the pyruvate dehydrogenase complex: A model for an assay using a conjugate with one ligand per subunit
✍ Alynne I. MacLean; Leonidas G. Bachas πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 581 KB

A homogeneous enzyme immunoassay for lipoic acid was developed by using an enzyme-ligand conjugate containing only one ligand per enzyme subunit. Theoretical studies have shown that the traditional use of multisubstituted enzyme-ligand conjugates has limited the detection limits and sensitivity obta