Purification, stabilization, and crystallization of a modular protein: Grb2

✍ Scribed by J. P. Guilloteau; N. Fromage; M. Ries-Kautt; S. Reboul; D. Bocquet; H. Dubois; D. Faucher; C. Colonna; A. Ducruix; J. Becquart

• Book ID: 102648380
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 906 KB
• Volume: 25
• Category: Article
• ISSN: 0887-3585
• DOI: 10.1002/(sici)1097-0134(199605)25:1<112::aid-prot9>3.0.co;2-l

✦ Synopsis

We report here the purification and the crystallization of the modular protein Grb2. The protein was expressed as a fusion with glutathione-S-transferase and purified by affinity chromatography on glutathione agarose. It was apparent from reverse phase chromatography that the purified protein was conformationally unstable. Instability was overcome by the addition of 100 mM arginine to the buffers. Because Grb2 appeared to be extremely sensitive to oxidation, crystallization experiments were performed with a dialysis button technique involving daily addition of fresh DTT to the reservoirs. The presence of 8 to 14% glycerol was necessary to obtain monocrystals. These results are discussed i n relation with the modular nature of Grb2.