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A simple strategy towards membrane protein purification and crystallization

✍ Scribed by Damian Niegowski; Marie Hedrén; Pär Nordlund; Said Eshaghi

Book ID
116541677
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
286 KB
Volume
39
Category
Article
ISSN
0141-8130

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📜 SIMILAR VOLUMES

Strategy for membrane protein crystalliz
Strategy for membrane protein crystallization exemplified with OmpA and OmpX
✍ Alex Pautsch; Joachim Vogt; Kirstin Model; Christian Siebold; Georg E. Schulz 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 239 KB 👁 1 views

The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bod

Purification, stabilization, and crystal
Purification, stabilization, and crystallization of a modular protein: Grb2
✍ J. P. Guilloteau; N. Fromage; M. Ries-Kautt; S. Reboul; D. Bocquet; H. Dubois; D 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 906 KB

We report here the purification and the crystallization of the modular protein Grb2. The protein was expressed as a fusion with glutathione-S-transferase and purified by affinity chromatography on glutathione agarose. It was apparent from reverse phase chromatography that the purified protein was co