## Abstract An efficient affinity chromatographic matrix based on chitosan for wheat germ agglutinin (WGA) purification was developed. The matrices assayed consisted of chitosan miniβspheres crossβlinked with epichlorhydrin 45, 250 or 500βmM. The maximum adsorption capacity of pure WGA β calculated
Purification of wheat germ agglutinin by affinity chromatography
β Scribed by Joel H. Shaper; Robert Barker; Robert L. Hill
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 395 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
Affinity purification is by tradition almost equivalent to affinity chromatography. In this report, a purification process is described in which the affinity interaction is performed in free solution. A precipitation step follows, thereby separating the affinity bound material from free. Chitosan is
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