✦ LIBER ✦

Measurement of wheat germ agglutinin binding with a fluorescence microscope

✍ Scribed by Michael A. Model; Jennifer L. Reese; Gail C. Fraizer

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 172 KB
Volume: 75A
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.a.20787

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Electron microscope assessment of fertil

Electron microscope assessment of fertilization of rabbit ova treated with Concanavalin A and Wheat Germ Agglutinin

✍ Gordon, M. ;Dandekar, P. V. 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 427 KB

## Abstract Zonaless rabbit ova, exposed to Concanavalin A or Wheat Germ Agglutinin, then to uterine capacitated sperm produce pronuclear, 2 and 4 stage embryos that are indistinguishable from controls. Absence of cortical granules indicates that the ova were fertilized and not merely activated. Su

Purification of wheat germ agglutinin us

Purification of wheat germ agglutinin using affinity flocculation with chitosan and a subsequent centrifugation or flotation step

✍ C. Senstad; B. Mattiasson 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 648 KB

Affinity purification is by tradition almost equivalent to affinity chromatography. In this report, a purification process is described in which the affinity interaction is performed in free solution. A precipitation step follows, thereby separating the affinity bound material from free. Chitosan is

Automated measurement of proteinase acti

Automated measurement of proteinase activity with a fluorogenic substrate using an inverted fluorescence microscope

✍ M.H. Dresden; J.P. Rotmans; A.M. Deelder; G. Koper; J.S. Ploem 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 289 KB

An automated method for measurement of proteinase activities using fluorogenic substrates is described. Enzyme assays were performed' in polystyrene microtitration trays as normally used for the enzyme-linked immunosorbent assay technique. The reaction products were measured using an inverted fluore

Origin of sympathetic and sensory innerv

Origin of sympathetic and sensory innervation of the elbow joint in the rat: A retrograde axonal tracing study with wheat germ agglutinin conjugated horseradish peroxidase

✍ B. Widenfalk; L.-G. Elfvin; M. Wiberg 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 590 KB

The wheat germ agglutinin-fractionated p

The wheat germ agglutinin-fractionated proteome of subjects with Alzheimer's disease and mild cognitive impairment hippocampus and inferior parietal lobule: Implications for disease pathogenesis and progression

✍ Fabio Di Domenico; Joshua B. Owen; Rukhsana Sultana; Rena A. Sowell; Marzia Perl 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 331 KB

## Abstract Lectin affinity chromatography is a powerful separation technique that fractionates proteins by selectively binding to specific carbohydrate moieties characteristic of protein glycosylation type. Wheat germ agglutinin (WGA) selectively binds terminal N‐acetylglucosamine (O‐GlcNAc) and s

A Method to Measure the Interaction of R

A Method to Measure the Interaction of Rac/Cdc42 with Their Binding Partners Using Fluorescence Resonance Energy Transfer between Mutants of Green Fluorescent Protein

✍ Debbie L. Graham; Peter N. Lowe; Peter A. Chalk 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 109 KB

Enhanced blue fluorescent protein (EBFP) and enhanced green fluorescent protein (EGFP) mutants of GFP in close proximity to one another can act as a fluorescence resonance energy transfer (FRET) pair. Unstructured amino acid linkers of varying length were inserted between EBFP and EGFP, revealing th