Purification of two proteinases from Aspergillus terreus by affinity chromatography
β Scribed by Miglena E. Stefanova
- Book ID
- 107781883
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 543 KB
- Volume
- 639
- Category
- Article
- ISSN
- 1873-3778
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A simple two-step method for the purification of malic enzyme from bovine heart mitochondria in high yield is described. It consists of successive affinity chromatography steps on immobilized C8-(aminohexyl)-NADP and W-(aminohexyl)-ADP. The molecular weight estimated by gel filtration of the homoge