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Purification of the (Na+ + K+)-adenosine triphosphatase from human renal tissue

โœ Scribed by J.Mark Braughler; Clinton N. Corder


Book ID
115734624
Publisher
Elsevier Science
Year
1977
Weight
850 KB
Volume
481
Category
Article
ISSN
0005-2744

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Purification of cardiac (Na+,K+)-activat
โœ Takahide Watanabe; Yuko Tawada; Munekazu Shigekawa ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 436 KB

A procedure is described for preparation of highly active (Na+,K+)-ATPax from rat heart which has a specific activity of 200-600 amol PJmg/h. The procedure is simple and can be applied to small amounts of heart muscle. (-1 g). The ATPase activity was more than 90% sensitive to ouahain (at concentrat