𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification of cardiac (Na+,K+)-activated adenosine triphosphatase from rat

✍ Scribed by Takahide Watanabe; Yuko Tawada; Munekazu Shigekawa

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
436 KB
Volume
175
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A procedure is described for preparation of highly active (Na+,K+)-ATPax from rat heart which has a specific activity of 200-600 amol PJmg/h. The procedure is simple and can be applied to small amounts of heart muscle. (-1 g). The ATPase activity was more than 90% sensitive to ouahain (at concentrations up to 1 mM). The ouabain sensitivity is biphasic with about 20% of the ATPase activity being inhibited at -3 X lo-' M ouabain. Q 1988 Academic Pits, Inc.

πŸ“œ SIMILAR VOLUMES

Characterization of gill (Na+ K)-activat
Characterization of gill (Na+ K)-activated adenosine triphosphatase from chinook salmon,Oncorhynchus tshawytscha
✍ Johnson, S. L. ;Ewing, R. D. ;Lichatowich, J. A. πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 891 KB

## Abstract (Na ^+^K)‐activated ATPase activity from gills of yearling spring chinook was examined using a new rapid assay method. Characterization of the enzyme activity was performed. Optimal activity was obtained at pH 7.2 in the presence of 240 mM NaCl, 120 mM KCl, 20 mM MgCl~2~ and 10 mM Na~2~