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Purification of the mitochondrial calcium uniporter from beef heart and characterization of its properties

✍ Scribed by Gritsenko, E. N. ;Kutyshenko, V. P. ;Saris, N. -E. L. ;Wahlsten, M. ;Jokela, J. ;Mironova, G. D.


Book ID
110157294
Publisher
Biophysical Society of Japan
Year
2010
Tongue
English
Weight
175 KB
Volume
55
Category
Article
ISSN
1349-2942

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Soluble, oligomycin-insensitive ATPase released from beef heart mitchondria by chloroform extraction can be further purified by Sepharose 6B gel filtration. This purification increases enzyme activity 4--5 times (100-130U/mg). According to specific activity, high purity and ability to reconstitute o