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Purification of soluble and membrane-bound proteases with substrate-analogous inhibitors by affinity chromatography

✍ Scribed by Günther Jahreis; Klaus Peters; Heidrun Kirschke


Book ID
117331148
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
191 KB
Volume
49
Category
Article
ISSN
0165-022X

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Val-D-Leu-Pro-Phe-Phe-Val-D-Leu, a specific inhibitor of aspartate proteinases of the pepsin type, was synthesized. Its bonding to activated 6-aminohexanoic acid-Sepharose 4B afforded an affinity support suitable for the purification of human, porcine, and chicken pepsin, human gastricsin, and bovin